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hcm hepatocyte culture medium bullet kit  (Lonza)


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    Lonza hcm hepatocyte culture medium bullet kit
    Hcm Hepatocyte Culture Medium Bullet Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcm hepatocyte culture medium bullet kit/product/Lonza
    Average 90 stars, based on 1 article reviews
    hcm hepatocyte culture medium bullet kit - by Bioz Stars, 2026-02
    90/100 stars

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    iPSCs from the CIRM (n=42), POST (n=34), and UCSF (n=12) cohorts were incubated with either 100μM oleate or BSA control for 24hr after which cells were stained with Nile Red and fluorescence levels quantified by FACS. A) Raw fluorescence values of BSA vs. oleate-treated iPSCs. P-values were calculated using a paired Student’s t-test. B) Comparison of oleate-induced intracellular lipid accumulation in all three cohorts. Dashed line indicates the value of oleate-induced lipid accumulation used to define the iPSC-based MASLD risk score.

    Journal: bioRxiv

    Article Title: Predicting Metabolic Dysfunction Associated Steatotic Liver Disease Risk Using Patient-Derived Induced Pluripotent Stem Cells

    doi: 10.1101/2025.01.13.632567

    Figure Lengend Snippet: iPSCs from the CIRM (n=42), POST (n=34), and UCSF (n=12) cohorts were incubated with either 100μM oleate or BSA control for 24hr after which cells were stained with Nile Red and fluorescence levels quantified by FACS. A) Raw fluorescence values of BSA vs. oleate-treated iPSCs. P-values were calculated using a paired Student’s t-test. B) Comparison of oleate-induced intracellular lipid accumulation in all three cohorts. Dashed line indicates the value of oleate-induced lipid accumulation used to define the iPSC-based MASLD risk score.

    Article Snippet: Briefly, cells were cultured in BSA-free HCM bullet kit media (Lonza) containing 100μM oleate conjugated to BSA, or fatty acid-free BSA as a negative control for 24 hours and stained with 100ug/mL Nile Red.

    Techniques: Incubation, Control, Staining, Fluorescence, Comparison